Why do we place the leaf in boiling water?

Question: Why do we place the leaf in boiling water?

Boiling the leaf in water is done to soften the leaf's tissues and dissolve the waxy cuticle on its surface, making it more permeable to the alcohol that will be used in subsequent steps of the experiment. The heat from boiling also denatures or breaks down enzymes that could potentially interfere with the experiment. By softening the leaf's tissues and dissolving the cuticle, the leaf can more effectively release its pigments into the alcohol, allowing for a more accurate and efficient experiment. In short, boiling the leaf in water is done to soften its tissues and dissolve its cuticle, making it more permeable to the alcohol used in the experiment.

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