Question: Describe how to test for protein in a food sample?
To test for protein in a food sample, several methods can be employed. One commonly used technique is the Biuret test. In this test, a small portion of the food sample is mixed with a solution of copper sulfate and sodium hydroxide. The presence of protein in the sample causes a color change from blue to purple or pink, indicating a positive result. Another method is the Bradford assay, where a food sample is mixed with a dye called Coomassie Brilliant Blue, which binds to proteins and produces a color change. The intensity of the color change can be measured using a spectrophotometer, providing an estimate of the protein concentration in the sample. These tests provide reliable and qualitative or quantitative information about the protein content in food samples, aiding in nutritional analysis and quality control processes.
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