What is the purpose of setting up a reaction without template dna when performing pcr?

Question: What is the purpose of setting up a reaction without template dna when performing pcr?

The purpose of setting up a reaction without template DNA when performing PCR is to serve as a negative control to detect any contamination or errors that may occur during the PCR process. The absence of DNA in the negative control should result in no amplification, and any observed amplification can indicate contamination or other issues, such as non-specific primer binding. The negative control serves as a baseline for comparison to the experimental samples, and can ensure the reliability and accuracy of the PCR results.

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अज्ञेय का वैवाहिक जीवन उतार-चढ़ाव भरा था, वर्णन कीजिए? Aage ka vaivahik jivan utar chadhav bhara tha varnan kijiye

सवाल: अज्ञेय का वैवाहिक जीवन उतार-चढ़ाव भरा था, वर्णन कीजिए? अज्ञेय का वैवाहिक जीवन उतार-चढ़ाव भरा था। उन्होंने दो विवाह किए, लेकिन दोनों विवाह सफल नहीं हो पाए। उनका पहला विवाह 1937 में राजकुमारी रत्नलता से हुआ था। यह विवाह अज्ञेय के परिवार की इच्छा के विरुद्ध था। अज्ञेय के पिता हीरानंद वात्स्यायन संस्कृत के विद्वान थे और वे चाहते थे कि अज्ञेय एक योग्य विदुषी से विवाह करें। रत्नलता एक साधारण परिवार से थीं और वे संस्कृत नहीं जानती थीं। इस कारण से, अज्ञेय के परिवार वालों ने इस विवाह का विरोध किया। अज्ञेय और रत्नलता का विवाह कुछ वर्षों तक चलता रहा, लेकिन अंततः उनका तलाक हो गया। इस तलाक के कई कारण थे, जिनमें अज्ञेय की व्यस्त जीवनशैली, रत्नलता की शिक्षा और सामाजिक पृष्ठभूमि में अंतर, और अज्ञेय के परिवार का विरोध शामिल था। अज्ञेय का दूसरा विवाह 1956 में कपिला मलिक से हुआ था। कपिला एक मशहूर संगीतकार थीं और वे अज्ञेय की साहित्यिक प्रतिभा की प्रशंसक थीं। यह विवाह कुछ वर्षों तक चलता रहा, लेकिन अंततः यह भी विफल हो गया। इस विफलता के कारणों में अज्ञेय के व्यस्त जीवनशैली, कपिला की स्वतंत्रताप्रियता,...

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